Comparison of L1 consensus primers with E6 type specific primers for detection of human papillomaviruses in paraffin sections of cervical neoplasia

In our comparative study of L1 consensus primers with E6 type-specific primers for detection of human papillomavirus (HPVs) by polymerase chain reaction (PCR) in 35 cases of cervical neoplasia, the detection rate by E6 primers (54%; 19/35) was significantly higher than that by L1 primers (25%; 9/35) (p < 0.01). And all specimens HPV-positive with L1 primers were also positive by E6 primers. HPV DNA could be amplified in 36% (9 of 25) of tissue by L1 consensus primers from which beta-globin gene was amplified as compared with 64% (16 of 25) of tissue by E6 type-specific primers. With the L1 consensus primers, 8 cases were positive for HPV-16 and 1 case was positive for HPV-33. These results show that the L1 consensus primers have inferior sensitivity to the E6 type-specific primers for the detection of HPV by PCR. But the L1 consensus primers have great value in making simultaneous detection of various HPV types in a single tube reaction, thus they permit reduction of time and the economic burden of the experiment.

Studies on intestinal trematodes in Korea V. A human case infected by Fibricola seoulensis (Trematoda: Diplostomatidae)

A 25-year old Korean male was admitted to Seoul National University Hospital on July 5, 1982 because of fever, epigastric discomfort and diarrhea. Laboratory examination revealed moderate eosinophilia, and large operculated helminth eggs in stool repeatedly. Other laboratory test resulted in normal range and bacteriological cultures were nefative. Under the impression of intestinal fluke infection, bithionol treatment with magnesium purgation was tried. From the diarrheal stool after treatment, a total of 79 small adult trematodes was collected, and identified as Fibricola seoulensis. The patient had eaten raw viscera of 2 snakes 9 days before admission in a rural village in Korea. It was assumed that this is the first record that described human infection by an adult diplostomatid fluke.

Segmentary Mobility of the Lumbar Spine in the Diagnosis of Low Back Pain / 대한정형외과학회잡지

Segmentary mobility of the mobile lumbar spines upon a total of 90 cases was studied by means of the functional radiographic examination. Segmentary mobility was determined by Falconer method. The cases were divided into four groups as Group I, 20 cases, who had no symptoms on low back, Group II, 20 cases, who had herniated disc in L4-5 confirmed either by surgery or by myelography, Group III, 30 cases, who had low back pain and neurological signs of L5 root irritation, and Group IV, 20 cases, who had low back pain as well as neurological signs of S1 root irritation. The result of the analysis was concluded as follows: 1. Total mobility of the lumbar spine in group I was significantly higher than other groups. Large normal variation in segmentary mobility of the lumbar spine was found, however, the mean angle of the segmentary mobility was highest in L4-5 and higher in the order of L5-S1, L4-3, L3-2 and L2-1. 2. Total mobility of the lumbar spine in group II was markedly reduced compared with group I, however, proportion of the segmentary mobility of L4-5 versus total mobility of the lumbar spine was somewhat higher than that of group 3. Total mobility of the lumbar spine in group III was significantly reduced than group I and significantly higher than group II. Proportion of the segmentary mobility of L4-5 versus total mobility of the lumbar spine in group III was somewhat higher than group I and the same as group II. 4. Total mobility of the lumbar spine in group IV was reduced than group I and group III, and higher than group II. Proportion of the segmentary mobility of L5-S1 was significantly higher than any other group. 5. In the groups with low back pain and herniated disc, in other words, there was found relatively increased segmentary mobility in the affected lumbar segments in contrast to reduced mobility of the total lumbar segments.

A Case Report of Total Hip Prosthesis / 대한정형외과학회잡지

The indication of the total hip prosthesis seems to be far less incidence in this country than western part of the world. Accordingly the report of this surgery is few in Korea. In this report a case of total hip prosthesis operation performed in December 1971 is described. The patient was a 41-year-old female who had marked limited motion of the left hip as well as walking disturbance in such a grade to manage only for toilet by help of bilateral axillary crutches. The hip had 40° of flexion contracture and total range of motion of the hip was 70. Diagnosis was an aseptic necrosis of the femoral head. McKee-Farrar type of total hip prosthetic operation was performed. Walking exercise started from seven weeks after the operation. She has been obtained markedly improved range of motion of the hip with total of 220° actively. 350° passively and normal walking ability. No complication occurred.